Fig. 3

TMED3 knockdown suppress osteosarcoma development in vivo. A In vivo imaging was performed to evaluate the tumor burden in mice of shTMED3 and shCtrl groups post tumor-inoculation; The fluorescence intensity was scanned and used as a representation of tumor burden in mice of shTMED3 and shCtrl groups. B, C MNNG/HOS cells with or without TMED3 knockdown, the volume (B) and weight (C) of tumors formed in mice was measured and calculated at indicated time intervals. (D) The Ki67 level in tumors removed from mice was detected by IHC as a representation of tumor growth. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001