Fig. 1

NEDD4L may be an E3 ligase for UBE2T. A Bioinformatics analysis came from http://ubibrowser.ncpsb.org.cn/ubibrowser/. B Among top five E3 ligase proteins, NEDD4L was extremely negative correlation with UBE2T, and data from http://gepia.cancer-pku.cn/. C NEDD4 family and UBE2T protein levels in LUAD tissues and adjacent normal tissues were analyzed by immunoblotting (IB) analysis. D, F Bar graph showing expression values of the identified genes using the study dataset, and data came from http://ualcan.path.uab.edu. The 95% confidence interval was also displayed. E, G Protein expression of NEDD4L and UBE2T in LUAD and their relationship with patient survival: Kaplan–Meier survival analysis indicated that patient with higher expression of NEDD4L was related to a better overall survival (log-rank test, P = 0.045) (E); While higher expression of UBE2T was related to a worse overall patient survival (log-rank test, P = 0.0011) (G)