Fig. 1

Schematic diagram of the study. Potential target genes were predicted and disease target genes were screened according to the structure of Que. Flow cytometry was used to detect the apoptotic effect of Que on breast tumor cells and IC₅₀ was obtained by MTT method. PBMCs were isolated from peripheral blood of normal subjects by density gradient centrifugation. In vitro amplification was performed using anti-human TCR γδ monoclonal antibody and Que intervention. Flow cytometry was used to detect the changes of γδ T cell subsets. Que and γδ T cells were co-cultured with breast tumor cells, and the specific killing of γδ T cells on breast tumor cells was determined by flow cytometry. Finally, western blot was used to detect the expression of potentially related JAK/STAT1 signaling pathway proteins between Que and prebreast cancer