Fig. 5

The co-activation of STAT3 and NF-κB is involved in the PAR1 activation-induced tumor promoting effect, while only NF-κB participates in the PAR4 activation-induced tumor inhibitory effect in ESCC. A, C, E Western blot analyzed the expression of p-STAT3/p-NF-κB in nucleoprotein and STAT3/ NF-κB in total protein of Kyse140 cells. B, D, F Immunofluorescence staining and confocal microscopy detected p-STAT3, STAT3 and p-NF-κB expression in Kyse140 cells. G MTT detected the effect of STAT3 inhibitor on Kyse140 proliferation. H Pulmonary metastasis was observed and recorded 50 days after Kyse 140 cells injection via tail vein. Average number of lung metastasis in each group was shown. Data are presented as mean ± SD from three independent experiments; comparison between two groups, *P < 0.05; **P < 0.01; ***P < 0.001