Fig. 4

CircTUBGCP3 acted as a sponge of mR-885-3p in LAC cells. A Identification of circTUBGCP3 specific binding with 5 miRNAs. B The luciferase activity of circTUBGCP3 3’UTR after the treatment with 5 miRNAs in HEK293T. C The binding sites between WT or Mut circTUBGCP3 3’UTR and miR-885-3p. D The luciferase activity of circTUBGCP3 3’UTR after the treatment with miR-885-3p mimic in A549 and SPC-A1. E RIP assay analysis of the binding between circTUBGCP3 or miR-885-3p and Ago2 protein in A549 and SPC-A1. F qPCR analysis of the expression levels of miR-885-3p after the transfection with circTUBGCP3 plasmids in A549 and SPC-A1. G FISH analysis of the co-localization of circTUBGCP3 with miR-885-3p in A549. H qPCR analysis indicated the downregulation of miR-885-3p and its negative correlation with circTUBGCP3 in LAC tissues. I TCGA cohort validated the downregulation of miR-885-3p in 419 LAC tissues and Kaplan Meier analysis of the association of high or low miR-885-3p expression with overall survival in LAC patients. Data are the means ± SEM of three experiments. **P < 0.01