Fig. 2

Induction effect of ALO on the apoptosis of NSCLC cells via intrinsic apoptotic pathway. A H1299 and A549 were treated with ALO for 24 h, and then were stained with Annexin V-FITC/PI, then analyzed by flow cytometry. Annexin V labeled with FITC used to identify early apoptotic cells, PI used to distinguish survival early cells from necrotic or late apoptotic cells. Apoptosis rate means the ratio of the amount of early and late apoptotic cells to the total amount of cells. *P < 0.05, compared to the control group (0 mM, solvent ethanol); #P < 0.05, compared to the ALO group (0.4 mM or 0.3 mM). B H1299 and A549 were treated with ALO for 24 h, and then cells were lysed with RIPA buffer. The cell lysates were analyzed by Western blotting with the indicated antibodies. *P < 0.05, compared to the control group (0 mM, solvent ethanol); #P < 0.05, compared to the ALO group (0.4 mM or 0.3 mM). Data are from one experiment representative of three independent experiments. Two-tailed Student’s test