Fig. 3
Mortalin is degraded by salvianolic acid B, a caffeic acid phenethyl ester analog, which is found to combine with mortalin. A Prediction of the binding of Sal B and mortalin by SYBYL-X software. B HCCLM3 cells were treated with 0–600 μM Sal B for 24 h or 48 h. Cell viability of the HCCLM3 cells was measured, *P < 0.05, statistically significant difference vs. 0 μM Sal B group. C 10 μM Sal B was used as a positive control and sterilized double-steamed water as a negative control. The retention time of Sal B was 4.88 min, the accurate mass number m/z was 717.1459, and the area was 9.7 × 104. Red marker represents the detection of Sal B samples. D After treatment with Sal B, cells were harvested for ubiquitination analysis of mortalin by immunoprecipitation. E Western blots of mortalin expression after pretreatment with MG132. After pretreatment with 20 μM proteasome inhibitor MG132 for 2 h, HCCLM3 cells were treated with Sal B for 48 h, and then cells were harvested for western blot analysis