Fig. 3

MicroRNA-155-5p directly targets 3′UTR of SOX1 and inhibits expression of SOX1, and is overexpression in CCA tissues. A Targetscan was used to screen candidate miRNAs regulating SOX1. B SOX1 protein levels in CCA cells with overexpression or inhibition of candidate miRNAs. C The relative mRNA level of SOX1 and miR-155-5p were determined respectively in TFK-1 and HUCCT-1 cells transfected with miR-NC, miR-155-5p and miR-155-5pI by RT-PCR. *p  < 0.05, **p  < 0.01, *** p  < 0.001. D The relative levels of miR-155-5p expression in four normal bile duct tissues and nine CCA tissues was analysis by RT-PCR. ****p  < 0.0001. E Box plots of miR-155-5p expression in CCA tissues compared to normal bile duct tissues. Data sourced from GEO databases GSE32957. ****p  < 0.0001. F Schematic illustration of the potential biding motifs for miR-155-5p in the wild-type (WT) 3′-UTR of SOX1 and their mutant-type (MT). G The plasmid map of psiCHECK2 which contained a SOX1 3′UTR-WT predicted binding site. H Relative activity of luciferase reporters with SOX1 3′UTR-WT and SOX1 3′UTR-MT after co-transfection with miR-155-5p mimics in HEK-293 T cells. ***p  < 0.001