Fig. 3

GLA inhibits STAT3 activation in human MM cells. A The six MM cell lines (RPMI-8226, OPM2, OCI-MY5, LP1, U266 and KMS11) were treated with GLA (10 μM) for 24 h, and the protein levels of p-STAT3, total STAT3 and PARP were detected by western blot assays. B RPMI-8226 and LP1 cells were treated with the indicated concentrations of GLA for 24 h, and the protein levels of p-STAT3 and total STAT3 were detected by western blot assays. C RPMI-8226 cells were treated with GLA at various time, and the protein levels of p-STAT3 and total STAT3 were detected by western blot assays. D HEK293T cells were transfected with Myc-STAT3 and/or Flag-STAT3 for 40 h, followed by GLA treatment for 8 h. Cell extracts were then prepared for immunoprecipitation (IP) with an anti-Flag antibody and subsequent western blot assay for Myc. E RPMI-8226 and LP1 cells were treated with GLA for 24 h, and then stimulated with IL-6 (50 ng/mL) for 15 min. The protein levels of p-STAT3 and total STAT3 were measured by western blot assay