Fig. 4

GLA activates apoptotic signaling pathway through STAT3 pathway. A RPMI-8226 cells were transfected with a STAT3 siRNA for 24 h followed by GLA treatment for another 24 h and then the cells were analyzed by flow cytometer. B RPMI-8226 cells were transfected with a STAT3 plasmid for 24 h, followed by GLA treatment for another 24 h and cells were then stained with Annexin V-FITC and PI and analyzed by flow cytometer. C RPMI-8226 cells were transfected with a STAT3 siRNA for 24 h, followed by GLA treatment for another 24 h. Cells were then subject to western blot assay. D RPMI-8226 cells were transfected with a STAT3 plasmid for 24 h followed by GLA treatment for another 24 h. Cells were then applied for western blot assay