Fig. 6
GLA suppresses myeloma tumor growth in xenograft mice models. A Human MM cells RPMI-8226 were injected subcutaneously into nude mice with a density of 3 × 107 cells/site/mouse. When tumors were palpable, mice (n = 6/group) were intraperitoneally given GLA (30 mg/kg body weight) in PBS containing 10% Tween 80 and 10% DMSO daily for continuous 19 days. The image of nude mice xenograft tumors was captured after the treatment of GLA for 19 days. Tumor volumes (B) and mouse body weight (C) were monitored every two days. Data are shown as the means ± SD. D The tumor tissues extracted were prepared for western blot assay. The protein level of PARP, pro-Caspase-3, cleaved-Caspase-3, p-STAT3 and STAT3 were measured. E Quantitative analysis of the expression of cleaved PARP, cleaved Caspase 3, p-STAT3 and STAT3. F IHC analysis was used to visualize tumor proliferation maker Ki-67, PCNA, and apoptosis related genes cleaved PARP, cleaved Caspase 3, and STAT3 pathway key genes p-STAT3, STAT3. Scale bar, 20 μm