Fig. 4

M2 macrophages secrete CXCL13 to promote EMT in ccRCC cells. A In the presence or absence of anti-CXCL13 antibodies, MTS detects the effect of M2 macrophages on the proliferation of ACHN and Caki-1. B In the presence or absence of anti-CXCL13 antibodies, the scratch healing test detects the effect of M2 macrophages on the migration of ACHN and Caki-1. C In the presence or absence of anti-CXCL13 antibodies, the Transwell chamber test detects the effect of M2 macrophages on the migration and invasion of ACHN and Caki-1. D In the presence or absence of anti-CXCL13 antibodies or CXCL13, western blotting revealed the effect of M2 macrophages and CXCL13 on ACHN and Caki-1EMT (*P < 0.05)