Fig. 6

HMGB1/RAGE induced the expression of drug resistance protein via NF-kB pathway. A (a-1) Leukemia cells that stably transfected with NC shRNA and RAGE shRNA were pretreated with 50 ng/mL rHMGB1 for 24 h, then cells were co-cultured with 0.4 μM ADM for another 24 h. Western blotting was used to detect the expression of P-gp and MRP as well as the phosphorylation of P65. (a-2) Was the quantitative data of P-gp and /GAPDH in both leukemia cell lines. (a-3) Was the quantitative data of RAGE, ABCB1 and ABCC1/GAPDH in mRNA expression. B (b-1) Cells that stably transfected with shRNA-NC were pretreated with the small inhibitor of NF-κB, PDTC (1 μM) for 12 h, following the treatment of ADM (0.4 μM). Cells were subjected to western blotting to assess the expression of P-gp and MRP and the phosphorylation of P65. (b-2) Were the mRNA expression of P-gp and MRP of cells treated with PDTC compared to NC group. GAPDH was used as loading control. Data are the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01