Fig. 2

CircSEC24A significantly promoted the proliferation of pancreatic cancer cells. A The circSEC24A expression in PANC-1 and MIA CaPa-2 cells transfected with si-circSEC24A by qRT-PCR. B, C CCK-8 assays was used to detected the proliferation ability of pancreatic cancer cells after different treatments. D–F EDU assays evaluated the proliferation of cells after silencing circSEC24A expression. Representative images of were obtained at ×400 magnification. G, H Colony formation assays was performed to count and analysis the number of cells colony formations. I The expression of CDK4, CDK6 and Cyclin D1 in pancreatic cancer cells were detected by western blot. J Flow cytometry was used to analyse the effects of circSEC24A on PANC-1 and MIA CaPa-2 cell cycle progression