Fig. 6

ZMIZ2 expression was upregulated in different breast cancer subtype cells, and the knockdown of ZMIZ2 inhibited the malignant behaviors of MDA-MB-231 cells. A qRT-PCR revealed the upregulation of ZMIZ2 mRNA expression in different breast cancer subtype cells. B Western blot assay showed the upregulation of ZMIZ2 protein expression in different breast cancer subtype cells. C ZMIZ2 was successfully knocked down in 293 T cells by transfection. D qRT-PCR and western blot assays indicated that ZMIZ2 was successfully knocked down in MDA-MB-231 cells by transfection. E CCK8 assay showed that the cell viability of MDA-MB-231 cells was significantly suppressed after the knockdown of ZMIZ2. F–G Transwell assays confirmed that the knockdown of ZMIZ2 significantly inhibited the migration and invasion of MDA-MB-231 cells. Magnification: 100 × ; bar scale: 50 μm. H Cell cycle analysis revealed that the knockdown of ZMIZ2 induced G1 phase arrest. I Flow cytometry showed that the knockdown of ZMIZ2 promoted MDA-MB-231 cell apoptosis. *P < 0.05, **P < 0.01, and ***P < 0.001 when compared with the corresponding controls