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Fig. 4 | Cancer Cell International

Fig. 4

From: Imperatorin induces autophagy and G0/G1 phase arrest via PTEN-PI3K-AKT-mTOR/p21 signaling pathway in human osteosarcoma cells in vitro and in vivo

Fig. 4

IMP induced G0/G1 phase arrest in cell cycle of human osteosarcoma cells. A A visualized cell cycle pathway heatmap with relative gene expression level. B The expression level of key genes in cell cycle pathway of 143B cells exposed to various concentration of IMP were determined by RT-PCR assay. The cell cycle distribution map of 143B cells treated with blank control (C), 25 μM IMP (D), 125 μM IMP (E) detected by cytometry, and the semi-quantitative statistical chart of them (F). The cell cycle distribution map of U2OS cells treated with blank control (H), 25 μM IMP (I), 125 μM IMP (J) detected by cytometry, and the semi-quantitative statistical chart of them (K). The expression level of key proteins CDK6 and Cyclin D1 of cell cycle pathway in 143B cells (G) and U2OS cells (L). *p  < 0.05; **p  < 0.01; ***p  < 0.001, one-way ANOVA; data were mean  ±  SD.+ p < 0.05;++ p < 0.01; +++ p < 0.001, one-way ANOVA; data were mean ± SD

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Cancer Cell International

ISSN: 1475-2867

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