Fig. 2

Knockdown of SCUBE3 expression suppressed proliferation, promoted apoptosis, and induced cell cycle arrest in HCC cells. A MTT-assays were performed to evaluate the viability of negative control and cells expressing low levels of SCUBE3, and Colony formation assay using knockdown of SCUBE3 cells. B The proliferation rate of Bel7404 cells was reduced by SCUBE3 knockdown, which was observed using the Celigo cell counting assay. C EdU assays were used to determine the effect of SCUBE3 on the proliferation of Bel7404 cells. D Relative caspase activity measured by caspase 3/7 assay showed apoptosis in knockdown of SCUBE3 cells (shSCUBE3). E Effect of SCUBE3 on apoptosis was analyzed through flow cytometry using Annexin V-APC staining. F Cell cycle distribution after inhibition of SCUBE3 was analyzed through flow cytometry (n = 3,*p < 0.05, **p < 0.01, ***p < 0.001. ns, no significance, KD, knockdown, NC, negative control)