Fig. 2

miR-378d loss expression promotes 5-Fu and cisplatin resistance, monoclonal formation, and tumor sphere formation of ESCC cells. a, b Cell viability assay; the cells were treated with different concentrations of 5-Fu or cisplatin for 24 h and then treated with CCK8 for 30 min; the OD was detected at 450 nm. T test and ANOVA test were used for statistical differences analysis; five repeat wells were used per concentration, *P < 0.05, ** P < 0.01, ***P < 0.001. c Silencing of miR-378d promoted the monoclonal formation of TE-1 (P = 0.0238) and KYSE-150 (P = 0.0181) cells; each experiment was repeated thrice. d miR-378d-mimic transient transfection detected the colony formation; each experiment was repeated thrice. e Tumor sphere formation assays in KYSE-150 and TE-1 cells. The graph is the summarized data of three independent experiments, ***P < 0.001. Scale bar = 890 μm