Fig. 1
HYPERLINK "sps:id::fig1||locator::gr1||MediaObject::0" METTL3 regulates m6A level in LUAD patients and NSCLC cells. A Representative IHC images and IHC scores of m6A level in 21 LUAD tissues and adjacent normal tissues (Scale bar: 50 μm). B Expression of METTL3 in LUAD patients based on TCGA samples using UALCAN platform. C Expression of METTL3 in LUAD patients based on GEO datasets (GSE2514). D Representative IHC images and IHC scores of METTL3 in 21 LUAD tissues and adjacent normal tissues (Scale bar: 50 μm). E and F Relative mRNA levels measured by RT-qPCR and protein levels analyzed by western blot of METTL3 in NSCLC cell lines (H1975, A549, PC9, H322, H460, SPC-A1 and H1299), compared with normal human lung epithelia cells (BEAS-2B). G Quantitative analysis of the percentage of m6A content in LUAD cells (H1975 and PC9), compared with BEAS-2B cells. H Quantitative analysis of the percentage of m6A content in METTL3 stable knockdown H1975 and PC9 cells. I Quantitative analysis of the percentage of m6A content in METTL3 overexpression H322 cells. J Transfection efficiencies of METTL3 stable knockdown in H1975 and PC9 cells were confirmed by western blot. K The overexpression efficiency of METTL3 after 24 h transfection of METTL3 or vector plasmid in H322 cells. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant