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Fig. 2 | Cancer Cell International

Fig. 2

From: The synergistic anticancer effect of the bromodomain inhibitor OTX015 and histone deacetylase 6 inhibitor WT-161 in osteosarcoma

Fig. 2

Effect of OTX015/WT-161 on apoptosis and the cell cycle of OS cells in vitro. A Flow cytometry was used to assess the apoptosis of MG63/U2OS cells. FITC and PI reagents were used to treat OS cells that had been infected with OTX015 and/or WT-161 for 48 h. The percentage of apoptosis in various drug treatment groups was measured. B The expression of cleaved caspase 3 and cleaved caspase 9 protein in DMSO, OTX and/or WT-161. C The cell cycle distribution was analysed by flow cytometry. The percentage of OS cells in the G0/G1, S, and G2/M phases in the bar chart indicates that OTX015/WT-161 cooperatively arrests the cell cycle in the G1/S phase. D western blot analysis of cyclin-related proteins was performed in MG63 and U2OS cells. Actin was used as a loading control. Data are means ± SD (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001, compared to DMSO

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Cancer Cell International

ISSN: 1475-2867

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