Fig. 6

OTX015/WT-161 synergistically inhibited the growth of transplanted tumours. A OS cells were injected into the axilla of nude mice. After three days, four classes of nude mice were fed with the previously described materials: vehicle; vehicle; OTX015 (50 mg/kg); WT-161 (50 mg/kg); and OTX015 combined with WT-161 (same concentrations as for single agent). After two weeks of therapy, the morphology and size of xenografts excised from nude mice are shown in this picture. B Tumour volume changes every three days in the four treatment groups, as well as the final weight of the tumour. C Immunohistochemical analyses of tumour tissues for the expression of β-catenin, PTEN, and pGSK-3β. Scale bars represent 50 μm. D Summary of working model. *P < 0.05 and **P < 0.01