Fig. 5

Both SKOV3 and A2780 cells were treated with STS, niraparib or their combination. a, b After SKOV3 and A2780 cells were transfected with mRFP-GFP-LC3, punctate dots (green and red dots) were detected by laser confocal microscopy. Positive signals were defined if the cell had five or more LC3 puncta in the cytoplasm. Scale bar: 20 μm. c, d Phosphorylated Akt (p-Akt), p-mTOR, p62 and LC3 were detected by western blot. GAPDH protein was used as a loading control. Three independent experiments were performed. ***P < 0.001, **P < 0.01, *P < 0.05