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Fig. 2 | Cancer Cell International

Fig. 2

From: DNA methyltransferase 1 inhibits microRNA-497 and elevates GPRC5A expression to promote chemotherapy resistance and metastasis in breast cancer

Fig. 2

Effect of DNMT1 on chemotherapy resistance and metastasis of breast cancer cells was examined. A DNMT1 expression in MCF-10A, MCF-7/ADR and MCF-7 cells evaluated using RT-qPCR. B Transduction efficiency in si-DNMT1 or oe-DNMT1-transduced cells determined using RT-qPCR. C The cell viability and IC50 value of ADR detected by CCK-8 assay. D Cell invasion ability detected by Transwell assay. E The migration ability of cells detected by scratch test, and the ratio of scratch area between 48 and 0 h used as the scratch healing rate. F Apoptosis in oe-DNMT1-transduced MCF-7 cells and in si-DNMT1-transduced MCF-7/ADR cells examined using flow cytometry. *p  < 0.05 vs. Oe-NC; &p  < 0.05 vs. si-NC. The experiment was repeated for 3 times independently

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Cancer Cell International

ISSN: 1475-2867

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