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Fig. 3 | Cancer Cell International

Fig. 3

From: DNA methyltransferase 1 inhibits microRNA-497 and elevates GPRC5A expression to promote chemotherapy resistance and metastasis in breast cancer

Fig. 3

Regulation of DNMT1 on miR-497 methylation. A The CpG island in the promoter region of miR-497 predicted by MethPrimer website. B The promoter methylation status in 3 cases of breast cancer tissues and adjacent normal tissues detected using MSP. M was the methylation status and U the non-methylation status. C Promoter methylation status in MCF-10A, MCF-7 and MCF-7/ADR cells monitored using MSP. D The methylation status of miR-497 promoter in oe-DNMT1-transduced MCF-10A cells or si-DNMT1-transduced MCF-7 cells examined by MSP. E The expression of miR-497 in oe-DNMT1-transduced MCF-10A cells or si-DNMT1-transduced MCF-7 cells determined using RT-qPCR. F The expression of miR-497 after the treatment of 5-aza-dc detected using RT-qPCR. G The degree of enrichment of DNMT1 on the miR-497 promoter after MCF-7 cells were treated with 5-aza-dc observed by ChIP assay. H The enrichment of H3K27me3 and H3K4me3 in miR-497 promoter region predicted by ENCODE database and validated by ChIP assay in MCF-7 cells. *p  < 0.05 between two groups. The experiment was repeated for 3 times independently

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Cancer Cell International

ISSN: 1475-2867

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