Fig. 5
miR-497 targets and downregulates GPRC5A to affect breast cancer chemotherapy resistance and metastasis. A Heat map of up-regulated gene expression in breast cancer dataset GSE33447. The abscissa represented the sample number, the ordinate the gene name, and the histogram on the upper right was the color scale. B The intersection of miR-497 target genes predicted by StarBase database, markedly up-regulated gene from GSE33447 dataset and up-regulated genes in breast cancer from GEPIA. The middle part represented the intersection of three sets of data. C The differential expression of GPRC5A in breast cancer and normal samples included in TCGA and GTEx. The red box plots in the figure represented tumor samples, and the gray box plots normal samples. D The binding site of miR-497 and GPRC5A predicted using StarBase database. E The binding of miR-497 and GPRC5A verified using dual-luciferase reporter gene assay. *p  < 0.05 vs. NC-mimic group. F The expression of GPRC5A after up-regulation of miR-497 in MCF-7/ADR cells or down-regulation of miR-497 in MCF-7 cells detected using RT-qPCR. *p  < 0.05 vs. NC-mimic-transduced MCF-7/ADR cells. &p  < 0.05 vs. NC-inhibitor-transduced MCF-7/ADR cells. G The viability and IC50 value measured using CCK-8. H Cell invasion detected by means of Transwell assay. I Cell migration examined using scratch assay and the ratio of the scratch area between 48 and 0 h used as the scratch healing rate. J Apoptosis detected using flow cytometry. K Drug resistance, apoptosis and EMT-related protein expression in each group of cells measured by immunoblotting. H–K The experiment was repeated for 3 times independently. *p  < 0.05 vs. NC-mimic  +  control. &p  < 0.05 vs. miR-497-mimic  +  control