Fig. 7

LMNB1 knockdown promoted the antitumor effects of PARPi in PRAD. A The knockdown efficiency of specific shRNAs targeting LMNB1 gene in 22Rv1 cells was validated by qRT-PCR. B Relative cell viability of LMNB1-deficient 22Rv1 cells exposed to a series of concentrations of olaparib was determined by CCK-8. C Western blotting was carried out to detect the levels of cleaved PARP and cleaved caspase3 in LMNB1 knockdown cells in the condition of olaparib treatment. D Differentially expressed HALLMARK gene sets with high LMNB1 expression in the TCGA PRAD cohort was screened out by GSEA. NES, normalized enrichment score. E The altered genes in the “DNA repair” pathway in the high LMNB1 group compared to low groups from the TCGA PRAD cohort were plotted