Fig. 2From: A role for the NPM1/PTPN14/YAP axis in mediating hypoxia-induced chemoresistance to sorafenib in hepatocellular carcinomaPTPN14 inhibits HCC growth and tumorigenesis in vitro and in vivo. The expression of PTPN14 in different HCC cell lines with varied metastatic potential was analyzed by qRT-PCR (A) and western blotting (B). C Verification of the efficiency of PTPN14 knockdown in HepG2 cells or overexpression in SK-Hep1 cells. D Cell proliferation was determined by MTT assays in HepG2 cells with stable PTPN14 knockdown and SK-Hep1 cells with PTPN14 stable overexpression at the indicated times. E Representative images and quantification of cell clones in HepG2 cells with stable PTPN14 knockdown and SK-Hep1 cells with PTPN14 stable overexpression as determined by a colony formation assay. HepG2 cells with stable PTPN14 knockdown and SK-Hep1 cells with PTPN14 stable overexpression were injected subcutaneously into nude mice (1 × 107 cells per mouse, five mice per group). Mice and tumors were evaluated 5 weeks post-injection and representative images are shown (F). Tumor growth curve during the 5-week study period (G) and tumor weights (H) are shown. Data are shown as the mean  ±  SEM. Statistically significant differences were determined by Student’s t tests. *p  < 0.05; **p  < 0.01 compared with the respective controlsBack to article page