Fig. 6

RUFY4 predicted immune infiltration and immunotherapy in a PDL1 related manner. A–D GSEA for the correlation between RUFY4 and TCR signaling pathway, cancer immunotherapy by PD-1 blockades, TCR and costimulatory signaling, and TCR costimulatory signaling. FDR < 25%, p < 0.05 was considered statistically significant. E Pathway network diagram established by clueGO based on the co-expressed genes of RUFY4. The color of each circle represents one type of signal pathway and its size represents the degree of statistical significance. Genes involved in each signal pathway are marked with red labels. F The mRNA levels of PDL1 in 22 ccRCC tissues and 5 adjacent normal tissues in ccRCC based on data from the Oncomine database. t-test, *p < 0.05. G The relationship between the expression level of RUFY4 and PDL1 based on the data from TCGA database. Spearman-test, p < 0.0001. H The relationship between the expression level of RUFY4 and the sensitivity of ICB immunotherapy. Chi-square tests were used to calculate the p-valve. The cut-off for classifying a patient into High/Low expression is the median of expression level. I The relationship between the expression level of RUFY4-PDL1 and the sensitivity of ICB immunotherapy. Low PDL1-RUFY4 means RUFY4 and PDL1 were both lower than the median expression level. Chi-square test were used to calculated the p-valve. J The difference of 8 immune cell proportions between low and high PDL1/RUFY4 groups. Low PDL1 + low RUFY4 means RUFY4 and PDL1 were both lower than the median of expression level. Chi-square tests were used to calculate the p-valve. **p < 0.01, ***p < 0.001, ****p < 0.0001. p = ns (no significance)