Fig. 6

MeCP2 knockdown enhanced 5-FU sensitivity in tumors in vivo. Xenograft tumors excised from mice inoculated with BGC823/5-FU cells stably expressing sh-MeCP2 or sh-con and treated with 5-FU or PBS. A Tumor images. B, C Tumor weight and volume (*p < 0.01, as compared with empty plasmid; ^#p < 0.01, as compared with MeCP2 overexpressing plasmid). D, E qRT-PCR for the mRNA levels of MeCP2 and NOX4 (*P < 0.05). F Western blot for the protein levels of MeCP2, NOX4 and PKM2. G. IHC staining of MeCP2