Fig. 1

The effect of SGLT2 inhibition on growth of TPC-1 and BCPAP cells. A, B Canagliflozin inhibited the viability of TPC-1 and BCPAP cells. TPC-1 and BCPAP cells were treated with 0, 5, 10, 20 μM canagliflozin for 48 h, then cell viability were measured by CCK8. One-way ANOVA were used to determine statistical significances, p < 0.0001. C, D Canagliflozin inhibited TPC-1 and BCPAP cells proliferation. TPC-1 and BCPAP cells were treated with 10 μM canagliflozin, then viable cells were measured at 0, 24, 48, 72, 96 h by CCK8. Repeated-measures analysis of variance were used to determine statistical significances, p < 0.0001. E, F Canagliflozin inhibited TPC-1 and BCPAP cells colony formation. TPC-1 and BCPAP cells were treated with 10 μM canagliflozin or dapagliflozin for 14 days, then colony formation was monitored by crystal violet stain. G Knockdown of SGLT2 inhibited the proliferation of TPC-1 cells. 24 h after transfection, the cells were plated into 96-well plates, and measured at 0, 24, 48, 72, 96 h by CCK8. Knockdown of SGLT2 in TPC-1 cells was verified by Western blot analysis. H Knockdown of SGLT2 inhibited cell viability of BCPAP cells. 24 h after transfection, cells were cultured in 96-well plates and stained with CCK8 at 72 h. Knockdown of SGLT2 in BCPAP cells was verified by western blot analysis