Fig. 5

KIF2A acted as a downstream target of miR-603. A Schematic representation of the putative binding sites between KIF2A and miR-603. B, C Dual-luciferase reporter assay was employed to determine the relationship between KIF2A and miR-603. D QRT-PCR presented the transfection efficiency of miR-603 mimic and inhibitor. E, F Influence of miR-603 overexpression or silencing on the levels of KIF2A mRNA and protein in HCC70 and MDA-MB-231 cells was analyzed by qRT-PCR or western blotting. G The level of KIF2A mRNA in BC tissues and adjacent non-cancerous tissues was assessed with qRT-PCR. H, I The correlation between KIF2A and miR-603 or circ_IRAK3 was assessed with Pearson’s correlation analysis. J Western blotting was used to analyze the level of KIF2A protein in BC tissues and adjacent non-cancerous tissues. K, L QRT-PCR and western blotting were performed to evaluate the levels of KIF2A mRNA and protein in BC cells and the MCF-10A cells. *P < 0.05