Fig. 4

STOX2 mitigates miR-30a stimulation of proliferation and metastasis in JAR cells. A and B CCK-8 assay showed the proliferation of JAR cells co-transfected with miR-30a mimics and STOX2 cDNA or miR-30a inhibitor and STOX2 siRNA. C and D Colony formation experiment assessing the growth of JAR cells. E and F The viability of JAR cells was analyzed using an EdU assay. The last line is the positive control. G and H Cell migration detected after co-transfection with STOX2 cDNA or miR-30a inhibitor and STOX2 siRNA into JAR cells. I and J Cell invasion detected after co-transfection with STOX2 cDNA or miR-30a inhibitor and STOX2 siRNA into JAR cells. Bar = 100 μm. *P < 0 .05 or **P < 0 .01, ***P < 0.001