Fig. 1

Role of PPT1 in HCC. A PPT1 mRNA expression in various normal human tissues and tumor tissues. B TCGA database-based comparison of PPT1 mRNA expression in HCC tissues (n = 371) and normal liver tissues (n = 50); ***P < 0.001. C Representative immunohistochemical results of PPT1 in patient-derived HCC tissue and normal liver tissue. Scale bar, 50 μm. D Expression of PPT1 in a panel of normal liver cell lines (L-O2 and MIHA) and HCC cell lines. On Western blot analysis, PPT1 was found to be preferentially expressed in HCC cell lines, Hep 3B and Hep 1-6. E Kaplan–Meier curves of overall survival (OS). High PPT1 expression was correlated with poor OS in HCC patients. F Cox proportional hazards regression model analysis of OS. G Gene set enrichment analysis (GSEA) using the TCGA dataset. PPT1 overexpression was significantly correlated with “KEGG_PATHWAYS_IN_CANCER”, “KEGG_APOPTOSIS”, “KEGG_REGULATION_OF_AUTOPHAGY”, and “KEGG_LYSOSOME” pathways. NES: normalized enrichmentscore