Fig. 6

Combined application of the CXCR2 inhibitor SB265610 and anti-PD-L1 antibody treatment significantly blocks HOXA7-promoted KRAS mutant CRC metastasis. A–E One week after the injection of CT26-HOXA7 cells, the mice in the four groups were treated with vehicle, PD-L1 antibody or/and SB265610 (n = 10 mice/group) until the treatment endpoint. In vivo assays showing that combined treatment with an anti-PD-L1 antibody and the CXCR2 inhibitor SB265610 almost completely blocked KRAS mutant CRC metastasis. A Representative bioluminescent images and bioluminescent signals in the indicated groups. B Overall survival in the indicated groups. C The numbers of lung and liver nodules in the indicated groups. D The incidence of lung and liver nodules in the indicated groups. E Representative HE staining of lung and liver tissues. F IHC staining showing the infiltration of MDSCs or CD8⁺ T cells of primary tumor in the indicated groups. All the data are presented as the mean ± s.d. *P < 0.05