Fig. 1

CARM1 and autophagy markers are increased in GC tissues, and higher CARM1 expression predicts worse prognosis. A Representative images of immunohistochemistry (IHC) staining of CARM1 in 48 GC tissues and 48 adjacent non-tumor tissues. (Scale bar: above, 500 μm; below, 50 μm). B CARM1 mRNA expression was significantly upregulated in GC tissues (n = 80) compared to normal tissues (n = 80) in the TCGA profile (Student’s t test, P < 0.001). C H-score of CARM1 in GC tissues and adjacent non-tumor tissues (n = 48) were evaluated by the staining intensity. (Student’s t test, P < 0.001). D Kaplan–Meier curve depicting the overall survival of GC patients (n = 48) stratified by high and low expression of CARM1 (log-rank test, P < 0.05). E Representative photographs of IHC staining of the autophagy markers ATG5, LC3B, and Beclin1 in 33 pairs of GC tissues and adjacent non-tumor tissues. (Scale bar: 50 μm). F The mRNA expression of ATG5, LC3B, and Beclin1 in 33 pairs of cancer and adjacent tissues was evaluated. G Western blot analysis of CARM1 protein expression in human GC cell lines and a gastric mucosal epithelial cell line. β-Actin was used as a loading control. H mRNA expression levels of CARM1 in four human GC cell lines and the GES-1 cell line were determined by qRT–PCR. Bars represent the mean ± SD from three independent experiments. *Represents Student's t test *P < 0.05, **P < 0.01 and ***P < 0.001