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Fig. 2 | Cancer Cell International

Fig. 2

From: CARM1 promotes gastric cancer progression by regulating TFE3 mediated autophagy enhancement through the cytoplasmic AMPK-mTOR and nuclear AMPK-CARM1-TFE3 signaling pathways

Fig. 2

CARM1 enhances autophagy of GC cells. A and B Knockdown of CARM1 in HGC27 cells and overexpression of CARM1 in BGC823 cells were confirmed by western blotting and qRT–PCR. C Representative images of autophagosomes and autolysosomes (indicated by the red arrows) by transmission electron microscopy. The images above are at a lower magnification (scale bar: 5 μm), and the images below are at a higher magnification (scale bar: 1 μm). D Representative photographs of immunofluorescence staining showing LC3B-positive cells (scale bar: 50 μm). E Protein levels of p62, LC3BII/I, ATG5, and Beclin1 were detected by western blotting using β-Actin as an internal control. (n = 3; error bar, SD). *Represents *P < 0.05, **P < 0.01 and ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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