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Fig. 8 | Cancer Cell International

Fig. 8

From: CARM1 promotes gastric cancer progression by regulating TFE3 mediated autophagy enhancement through the cytoplasmic AMPK-mTOR and nuclear AMPK-CARM1-TFE3 signaling pathways

Fig. 8

The TFE3 activity is activated via the cytoplasmic AMPK-mTOR and nuclear AMPK-CARM1-TFE3 signaling pathways. A Western blotting analysis of the AMPK-mTOR signaling pathway in the cytoplasm and TFE3 expression in the nucleus in the CARM1-EV, CARM1-OE, CARM1-OE + DMSO, and CARM1-OE + Compound C (10 μM, 6 h) cells. B CARM1-overexpressing BGC823cells were treated with Compound C (10 μM, 6 h) or the solvent control DMSO. An immunoprecipitation assay using a CARM1 antibody was performed, and the amount of TFE3 protein bound to CARM1 under different experimental conditions was evaluated by western blotting. Values represent the means ± SD. **P < 0.01 and ***P < 0.001. C Schematic model for the mechanisms of function of CARM1 in GC. CARM1 potentiates autophagy by facilitating TFE3 nuclear translocation and enhancing its activity by activating cytosolic AMPK-mTOR and nuclear AMPK-CARM1-TFE3 signaling pathways. Then, CARM1 promotes GC cell proliferation and reduces ER stress-induced apoptosis by regulating autophagy

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Cancer Cell International

ISSN: 1475-2867

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