Fig. 8

Rcl1 inhibits HCC cells proliferation, migration and invasion in vitro. A, B western blot analyses and C, D qRT-PCR assays of Rcl1 overexpression and knockdown in Huh-7 and Hep-3B cells. E, F CCK-8 assays of Huh-7 and Hep-3B cells after Rcl1 overexpression or knockdown with the corresponding negative controls. G, H Transwell assays examined the migration and invasion activities of Rcl1 overexpression or knockdown with their corresponding negative controls. Data are presented as the mean ± standard deviation from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001. Bar = 100 μm