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Fig. 2 | Cancer Cell International

Fig. 2

From: Novel sequential therapy with metformin enhances the effects of cisplatin in testicular germ cell tumours via YAP1 signalling

Fig. 2

A TCam-2 and NTERA-2 cells were treated with metformin for 48 h, and flow cytometry was used to assess the changes in the cell cycle during withdrawal at 24 and 48 h. B The viability of TCam-2 and NTERA-2 cells after exposure to the five treatment strategies was determined by CCK-8 assay. C The IC50 of cisplatin was determined in TCam-2 and NTERA-2 cells treated with combination treatment (metformin + cisplatin) and sequential treatment (metformin  − cisplatin). D A colony formation assay was used to detect the anticancer effects of the different treatment strategies. E Flow cytometry was used to detect the effects of apoptosis of the different treatment strategies. The average percentage of TCam-2 and NTERA-2 cell apoptosis was quantified

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Cancer Cell International

ISSN: 1475-2867

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