Fig. 3

A The differential expression of YAP1 in 139 tumours and 165 normal testicular tissues visualized by using RNA-seq data from TCGA and GTEx (a). The differential expression of YAP1 in 165 normal tissues and 62 seminomas (b). The differential expression of YAP1 in 53 non-seminomas and 62 seminomas (c). The differential expression of YAP1 in 165 normal tissues and 53 non-seminomas (d). B The mRNA expression of YAP1 in TCam-2 and NTERA-2 cells. C Immunofluorescence analysis was used to assess the expression of YAP1 in TCam-2 and NTERA-2 cells treated with metformin. Images were captured at 200 × with an Olympus microscope. The percentage of cells with YAP localized in the nucleus (n ≥ 30 cells). D–F. Sequential treatment with metformin and cisplatin enhanced the effects of cisplatin through the YAP1/CDK6/CDK4/cyclinD1/RB axis and Caspase 3. Western blotting was used to assess the expression of related proteins