Fig. 5

A Schedules of the four different treatment strategies. B Randomly grouped nude mice bearing TCam-2 and NTERA-2 tumours were treated with PBS (NC), Met (200 mg/kg), Cis (5 mg/kg) or metformin-cisplatin sequential treatment (Met-Cis) for three cycles. Representative tumours in nude mice from different groups. Tumour volumes were measured every 3 days, and the tumour weights were determined immediately after harvesting the whole tumours. C, D The expression levels of YAP1, P-YAP1, CDK6, CDK4, cyclinD1, RB, caspase 3 and cleaved caspase 3 were determined by Western blot in tumour tissues from the xenograft mice treated with different treatments