Fig. 1
Expression of DAB2IP in ESCC cell lines and tissues and its prognostic significance in ESCC patients. A The levels of DAB2IP protein in four ESCC cell lines and normal esophageal mucosa (N1 and N2) examined by Western blot (left); Western blot analysis of DAB2IP protein expression in the 10representative paired of ESCC (T) and normal esophageal mucosa (N) (right). B (a) Representative images showed strong DAB2IP IHC signaling of normal esophageal mucosa. (b) Low DAB2IP expression was detected in ESCC case #32 (total score = 1 + 1), with 15% (staining percentage score = 1) carcinoma cells demonstrated weak staining (staining intensity score = 1) of DAB2IP. (c) Moderate DAB2IP expression (total score = 6 + 2) in ESCC case #45, where about 60%-70% (staining percentage score = 6) carcinoma cells demonstrated moderate staining (staining intensity score = 2) of DAB2IP. (d) High expression (total score = 9 + 3) of DAB2IP was observed in ESCC case #91, with 100% (staining percentage score = 9) carcinoma cells demonstrated strong staining (staining intensity score = 3) of DAB2IP. C X-tile plots of the prognostic marker DAB2IP. X-tile analysis was conducted on patient data from our ESCC cohort. The plot showed the χ2 log-rank values created when the cohort was divided into two population. (a) The cut point highlighted by the black/white circle was demonstrated on the histogram. (b) DAB2IP expression was divided at the optimal cut point, as defined by the most significant on the plot. (c, d) Kaplan–Meier survival analysis of DAB2IP expression for OS (n = 140, (c)) and DSS (n = 112, (d)) in ESCC patients treated with definitive CRT (log-rank test)