Fig. 2

The impact of DAB2IP levels on the chemosensitivity of ESCC cells in vitro. A Shift of dose–response curves of cisplatin by overexpression or knockdown of DAB2IP was determined by MTS assay. Overexpression of DAB2IP in Kyse150 cells is significantly more sensitive to cisplatin than parental control cells (left). Consistently, knockdown of DAB2IP reduced sensitivity of EC109 cells to cisplatin (right). IC50 values were shown below. B Upper panels, Kyse150-DAB2IP and the control Kyse150-vector cells were treated with cisplatin (IC30), and 24 h later, the cells were collected and stained with PI and AnnexinV. Lower panels, EC109-shluc and EC109-shDAB2IP cells were incubated with cisplatin (IC30). After 24 h, Annexin V and PI staining assay was used to determine the percentage of cells undergoing apoptosis. The p-values were calculated by the Student’s t test. The bar shows the mean ± SD of three independent experiments. *p < 0.05. C Left, 24 h after cisplatin (IC30) treatment, overexpression of DAB2IP increased the levels of cleaved PARP and cleaved caspase-3 in Kyse150 cells. Right, silencing of DAB2IP decreased cisplatin-induced levels of cleaved PARP and cleaved caspase-3 in EC109 cells