Fig. 2

BFXJY treatment reduced the production of IL-1β and IL-18 in lung cancer with Qi-yin deficiency. A, B The serum level of IL-1β (A) and IL-18 (B) was detected using ELISA assay in lung cancer with Qi-yin deficiency. C–E The relative protein level of IL-1β (C and D) and IL-18 (C and E) was examined using western blot in lung cancer with Qi-yin deficiency. The data were expressed after being normalized to GAPDH. F, G The distribution of IL-1β (F and G) and IL-18 (F and H) was also evaluated by IHC. The means ± SD of three independent samples were shown. Differences among multiple groups were analyzed using one-way analysis of variance and followed Duncan's test using the SPSS 20.0 package. *p < 0.05 compared to the control group and ^#p < 0.05 compared to the Qi-yin deficiency + LPS-ATP group