Fig. 4

BFXJY treatment might inhibit PKC signaling in lung cancer with Qi-yin deficiency. A The serum level of MDA was detected using an ELISA assay. B–D The relative protein level of MDA (B and C) and PKC α (B and D) was examined using western blot in lung cancer with Qi-yin deficiency. The data were expressed after being normalized to GAPDH. E–G The distribution of MDA and PKC α was also evaluated by IHC. The means ± SD of three independent samples were shown. Differences among multiple groups were analyzed by one-way analysis of variance and followed Duncan's test using the SPSS 20.0 package. *p < 0.05 compared to control group and ^#p < 0.05 compared to the Qi-yin deficiency + LPS-ATP group