Fig. 3
From: PGM1 suppresses colorectal cancer cell migration and invasion by regulating the PI3K/AKT pathway
Successful construction of PGM1-overexpression and knockdown cell lines. A PGM1 expression in CRC cells as measured by RT-PCR. B PGM1 mRNA in control cells and in cells transfected with PGM1 shRNA. ***P < 0.01 vs. shCTRL. C PGM1 mRNA expression in cells transfected with pcDNA4.0 vector, pcDNA4.0-PGM1 vector, NC‑shRNA, or PGM1‑shRNA was determined by RT-qPCR. D, E PGM1 protein expression in the following five groups of transfected cells: CTRL, OE-NC, OE-PGM1, shCTRL, and shPGM1. F Images of cells transfected with the aforementioned vectors or shRNAs after being stained for PGM1 expression (red); nuclei are stained blue (DAPI). IOD, integrated optical density; CTRL, negative control; OE, over expression. Data are presented as a mean value ± SD of results obtained from three replicate samples; **P < 0.01, ***P < 0.001, ****P < 0.001; ##P < 0.01, ###P < 0.001