Fig. 2

Effects of ERK inhibitors or MEK inhibitors on expression of PD-L1 in iCCA cell lines. A, C Levels of ERK, p-ERK and PD-L1 were validated by western blotting in HuCCT1 and RBE cells treated by different concentrations (0.5, 1.0, 2.0, 4.0 μM) of ERKi or MEKi for 24 h. B, D Representative images of positive staining of PD-L1 (red) and DAPI (blue) was determined by immunofluorescence using confocal microscopy in HuCCT1 and RBE cells treated by ERKi (4 μM) or MEKi (4 μM) for 24 h (Scale bar, 150 μm)