Fig. 1

BMI1 affects OS cells proliferation and migration in a dose-dependent way. A OS cells were treated with PTC-209 for 48 h, followed by western blot analysis. Tubulin was used as an internal control. Sample size = 3. B Quantification of A. C After OS cells were treated with the indicated doses of PTC-209 for 48 h, the proliferative viability of OS cells was detected using CCK-8 assay. Sample size = 6. D Colony formation assays were used to detect the colony forming ability of OS cells after treated with the indicated doses of PTC-209 for 48 h. Sample size = 3. E Quantification of D. F After treated with the indicated doses of PTC-209 for 48 h, transwell assays were conducted to investigate migratory abilities of OS cells. Sample size = 3. G Quantification of F. *p < 0.05, **p < 0.01, ***p < 0.001, compared with control