Fig. 3
From: BMI1 promotes osteosarcoma proliferation and metastasis by repressing the transcription of SIK1
BMI1 affects 143B OS cells metastasis in vivo. A Cells treated with 10µM PTC-209 for 48 h and control cells were injected into the tail vein of nude mice. Lung tissues were took out and photographed. Sample size = 5. B Counting the number of lung nodules. C H&E staining was performed on paraffin sections of lung tissues. Sample size = 3. Scale bar: 500 μm. D-F Immunofluorescence staining of E-cadherin, N-cadherin, and Vimentin in control and PTC-209 treated group. Sample size = 3. Scale bar: 20 μm. G Quantification of D–F. ***p < 0.001, compared with control