Fig. 4

SIK1 is a potential downstream gene of BMI1. A, B Distribution of transcription factor-binding loci relative to Transcription Start Sites (TSS) in the indicated cell lines. C Bubble plot of functional enrichment: the color represents the p value, and the circle size represents the number of genes. D Network diagram of functional enrichment: purple circles represent function, blue circles represent gene, lines represent the presence of a relationship between gene and function. E A common transcription factor binding peak for BMI1 in the promoter region of SIK1 in the indicated cell lines. F After treated with the indicated doses of PTC-209, the relative expression of SIK1 in OS cells was detected by qRT-PCR. Sample size = 3. *p < 0.05, **p < 0.01, ***p < 0.001, compared with control